Fig. 7.

Osteogenic differentiation of BMSCs in HPL from PCs stored for 0–4 months (m). a ALP activity at 7 and 14 days (d) in osteogenic (+) and standard (−) HPL media. ALP activity (absorbance) was normalized to the corresponding DNA content (ng/mL). b Representative images and quantification of Alizarin Red S (ARS)-stained cells after 21 days of induction. Data represent means ± SD; each symbol represents a single donor (D, n = 4 donors) based on the average of 3 experimental replicates