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. Author manuscript; available in PMC: 2021 Aug 17.
Published in final edited form as: Chem Res Toxicol. 2020 Aug 4;33(8):2072–2086. doi: 10.1021/acs.chemrestox.0c00027

Figure 9.

Figure 9.

ROS detection using fluorescence and absorbance sensors. (A) Fluorescence intensity vs incubation time for DST assay for hydroxyl radical detection; (B) SOSG fluorescence assay for singlet oxygen detection. (C) LCV absorbance assay for H2O2 and (D) NBT assay for superoxide radical. DNA–HLuM films on magnetic bead reactors were incubated with 2 mM NNK or NNN + the NADPH regenerating system + 5 mM Cu2+ for different time intervals with reagents needed for respective detection assays. (A–C) Reactions with and without DNA in films on biocolloid reactors. The control for all assays was the absence of a cyt P450 enzyme source on magnetic beads in PBS buffer (pH 7.4).