FIGURE 4.

Multiplexing and reusability with PRADA. (a) Multiplexed detection of both cTnI and NPY in a three‐well microfluidic device. The concentrations measured in ng/ml for cTnI/NPY are 3000/1500 (blue), 1000/600 (red), 400/200 (black), 200/100 (orange), 5/10 (light blue), 0.5/1 (green), 0.05/0.3 (purple), and control (gray). The inset is a schematic representation of multiplexed detection of both biomarkers. (b) Image of a microfluidic device utilized in the experiments. (c) Demonstration of reusability of PRADA where the same microfluidic chip was reused 14 times after washing and regenerating. Here, 0.1 ng/ml of cTnI was detected at the DTNB Raman peak with minimal signal loss. Error bars indicate the standard deviations from at least five measurements. PRADA, portable reusable accurate diagnostics with nanostar antennas