FIGURE 2.

Growth of P. syringae after infiltration into plants overexpressing miR167a. (a) Phenotype of P_35S::MIR167a plants. Five‐week‐old Col‐0 and P_35S::MIR167a plants were photographed. White arrows indicate leaves with curled shape and leafy petioles that were used to identify P_35S::MIR167a plants during vegetative growth. Scale bars indicate 2 cm. (b) and (c) Five‐week‐old Col‐0 or P_35S::MIR167 plants were infiltrated with Pst DC3000 (b) or Pst DC3000 carrying avirulence factors (avrRpm1) or (avrRpt2) (c) at a titer of 5 x 10⁵ cfu/ml and bacterial growth was determined after 0 and 3 days (b) or 3 days only (c). Bars represent mean + SEM of pathogen growth. For all graphs, asterisks indicate significantly different growth from Col‐0 at p < .05 using the Wilcoxon rank‐sum test. Experiments were performed on eight to ten plants per genotype and each experiment was repeated three to five times with similar results. Results of one such experiment are shown. As P_35S::MIR167a plants are sterile, a population of independent T₁ transgenic plants was used rather than stable transgenic lines