FIGURE 8.

P_35s::MIR167 plants are unable to activate systemic acquired resistance. Three lower leaves of Col‐0 and P_35S::MIR167 plants were infiltrated with either 10 mM MgSO₄ (mock) or Pst DC3000 (avrRpm1) at 1 × 10⁸ cfu/ml to induce SAR. Two days later, secondary leaves were infiltrated with 5 × 10⁵ cfu/ml Pst DC3000. Bacterial growth was measured after 3 days. Bars represent means + SEM; different letters indicate statistically significant differences in pathogen counts (p < .05, Kruskal‐Wallis test followed by pairwise Wilcoxon rank‐sum tests using Hochberg p‐value adjustment). This experiment was performed on seven to ten plants of each genotype and was repeated three times with similar results. Results of one such experiment are shown. As P_35S::MIR167a plants are sterile, a population of independent T₁ transgenic plants was used rather than stable transgenic lines