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. 2020 Sep 9;7:571696. doi: 10.3389/fmolb.2020.571696

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Copyright © 2020 Feuillie, Lambert, Ewald, Azouz, Henry, Marsaudon, Cullin, Lecomte and Molinari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction between L34T mutant peptides and a POPC/SM/Chol/GM1 membrane. Successive HS-AFM height images extracted from video 4 before injection (A), 100 s after injection (B), 500 s after injection (C), 1000 s after injection (D), 2000 s after injection (E), and 3000 s after injection. The white circle indicates a same recognizable landmark in the scanned area to indicate a sight displacement of the scanned area toward the lower area over the course of the scan. Red and white arrows indicates L34T peptides in contact with the SLB or the mica substrate, respectively. One of the advantages of HS-AFM is the ability of moving the scanned area in real time, in the limits of the X and Y piezoelectric scanners, respectively ∼1 and 3.2 μm. This allows zooming in in real time to focus on details (as here in E).