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. 2020 Sep;61:79–88. doi: 10.1016/j.ymben.2020.05.005

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Effects of different gRNAs on relative transcription level, fatty acid content, and naringenin production. a - c Mechanism of gRNA1, gRNA2, and gRNA3 guided fatty acid synthesis auto-regulation. d - f Effects of different gRNA1, gRNA2, and gRNA3 on relative transcription level and fatty acid content. The relative transcription level of FAS1, FAS2, and FabD was normalized by the transcription level of FAS1, FAS2, and FabD without auto-regulation (the control). g - i Effects of repressing FAS1, FAS2, and FabD using different gRNA1, gRNA2, and gRNA3 on naringenin titer. Strain NarPro/ASC without auto-regulation circuit was used as control. gRNA1 refers to gRNA FAS1-1, FAS1-2, or FAS1-3. gRNA2 refers to gRNA FAS2-1, FAS2-2, or FAS2-3. gRNA3 refers to FabD-1, FabD-2, or FabD-3.