Fig. 4.

Combinatory repression analysis and time course of fatty acid and naringenin production. a Mechanism of auto-regulating fatty acid synthesis by combinatory repressing FAS1, FAS2, and/or FabD. b Effects of combinatory repressing FAS1, FAS2, and/or FabD on fatty acid accumulation and naringenin titer. c Analysis of the tradeoff between fatty acid and naringenin synthesis. The red line refers to the linear fit to the mean values. c Time course of fatty acid accumulation and naringenin production. Strain NarPro/ASC, which is identical to NarPro/ASC_Rep but without the negative auto-regulation circuit, was used as control. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)