Fig. 5.
Naringenin inducible promoter analysis. a Structures of the hybrid promoters. The hybrid promoters OTEF(111), OTEF(136), OLEU2(78), and OGAPDH(88) were composed of FdeR binding site FdeO and the core promoters of TEF(111), TEF(136), LEU2(78), and GAPDH(88) respectively. b Mechanism of the naringenin inducible transcription. PHybrid refers to the hybrid promoters of PO-TEF(111), PO-TEF(136), PO-LEU2(78), and PO-GAPDH(88). Nluc was used as the reporter gene. c The result of induction of the hybrid promoters. The luminescence was recorded using Cytation 3 microtiter plate reader. The RLU was calculated by integrating the read-out data by time.