FIGURE 6.
IL-12 and IFN-γ activate MAPK and NF-κB pathways. (A) MDMs (n = 6, similar results in an additional six donors) were treated with 10 ng/ml IFN-γ or 10 ng/ml IL-12 for 30 min. (Left) Representative flow cytometry of phosphoproteins with mean fluorescence intensity (MFI) values shown. (Right) Summary of fold phosphorylation of MAPKs and IκBα. (B) MDMs (n = 6, similar results in an additional eight donors) were transfected with scrambled or the indicated siRNA. Cells were treated with 10 ng/ml IFN-γ or 10 ng/ml IL-12 for 24 h. Cytokine secretion. Mean + SEM. Significance is shown compared with cytokine-treated, scrambled siRNA-transfected cells. *p < 0.05, **p < 0.01, ***p < 0.001, †p < 1 × 10−4, ††p < 1 × 10−5. scr, scrambled; Tx, treatment.