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. 2020 Sep 23;128(9):097006. doi: 10.1289/EHP6470

Figure 4.

Figure 4 is a set of two bar graphs, plotting Body Length (millimeter) and Yolk Sac Area (micrometer squared), ranging from 0 to 0.2 in increments of 0.2, 0.2 to 3.0 in increments of 2.5, and 3.0 to 4.0 in increments of 0.2 and 0 to 250000 in increments of 50000, respectively, (y-axis) across 0, 5.11, 10.23, 20.46, and milligram or liter polyfluoroalkyl substances mixture and 0, 1.14 times 10 begin superscript negative 5 end superscript, 2.81 times 10 begin superscript negative 3 end superscript, 5.63 times 10 begin superscript negative 3 end superscript, and relative percent aqueous film-forming foam (x-axis).

Measurements of larval development with exposure to a perfluorooctanesulfonic acid/perfluorohexanesulfonic acid (PFOS/PFHxS) mixture, at a ratio consistent with the legacy AFFF sample (6.21:1, PFOS:PFHxS). Larval body length (A) and yolk sac area (B) are reported as total PFAS concentration of the mixture and the relative % AFFF those mixtures represent. Control larvae were exposed to a 0.01% v/v DMSO solution. Bars represent mean±SEM. Asterisk (*) indicate p<0.05 compared with 0mg/L PFAS mixture, one-way ANOVA, Tukey’s post hoc test, n=26–45 larvae per treatment group. Note: AFFF, aqueous film-forming foam; ANOVA, analysis of variance; PFAS, per- and polyfluoroalkyl substances; PFOS, perfluorooctanesulfonic acid; PFHxS, perfluorohexanesulfonic acid.