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. 2020 Sep 23;15(9):e0239396. doi: 10.1371/journal.pone.0239396

Fig 3. TRI MP administration lowers level of anti-collagen II IgG antibodies.

Fig 3

A) Representative serial dilution curves with each curve corresponding to a single mouse, black–PBS treated mouse, red–Blank MP treated mouse, blue–TRI MP treated mouse, green–monoclonal anti-collagen II (CII) antibody (Ab) (Clone 2B1.5) used as standard. B) Normalized anti-CII IgG Ab titer versus arthritis score. Ab titer was defined as the dilution corresponding to the half-maximal absorbance in the linear section of the dilution curve, or the IC50 value using a non-linear four parameter regression. Normalized titer was calculated by dividing the titer by that of the 2B1.5 Ab standard for a given plate. Color coded based on treatment group: black–PBS, red–Blank MP, blue–TRI MP. Spearman correlation coefficient and p value for correlation are indicated. C) Average normalized anti-CII IgG Ab titer by treatment group. n = 12 mice per group, data presented as mean ± SEM, and the following cutoffs were used for significance: * p < 0.05.