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PCR master mix component and concentration.
| Multiplex mix | Volume (µL) | Final concentration |
|---|---|---|
| H2O PPI | 1.3 | |
| Reaction mix 2X | 12.50 | 3.0 mM Mg |
| MgSO4 (50mM) | 0.40 | 0.8 mM Mg |
| Forward Primer (10μM) | 1.00 | 0.4 μM |
| Reverse Primer (10μM) | 1.00 | 0.4 μM |
| Forward Primer (10μM) | 1.00 | 0.4 μM |
| Reverse Primer (10μM) | 1.00 | 0.4 μM |
| Probe (10μM) | 0.40 | 0.16 μM |
| Probe (10μM) | 0.40 | 0.16 μM |
| SupersriptIII RT/Platinum Taq Mix | 1.00 | |
| Final volume | 25.00 |
