Table 3.
RT-qPCR amplification cycles.
| Cycle | Temperature (°C) | Time | Number of cycles |
|---|---|---|---|
| Reverse transcription | 55°C | 20 min | X1 |
| Denaturation | 95°C | 3 min | X1 |
| Amplification | 58°C | 30 sec | X40 Acquisition |
| Cooling | 40°C | 30 sec | X1 |
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