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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Nat Cancer. 2020 Apr 20;1(5):533–545. doi: 10.1038/s43018-020-0056-0

Extended Data Fig. 5. The ISR pathway regulates PD-L1 expression independently of IFN-γ.

Extended Data Fig. 5.

a, Western blot analysis in H1944 cells expressing control or UROD sgRNA after treatment with 200 nM ISRIB for 24 h. b, Western blot analysis of UROD in H358 and H1944 cells treated with 10ng/mL IFN-γ for 24h. c, Western blot analysis of PD-L1 in H1944 cells treated with DMSO or 100uM Salubrinal for 24h +/− 10ng/mL IFN-γ (left) or 10uM Ruxolitinib (right). d, Western blot analysis of PD-L1 in H1944 cells expressing a control or UROD sgRNA +/− 10ng/mL IFN-γ (left) or 10uM Ruxolitinib (right). All experiments were performed three independent times with similar results. Representative data are shown.