Extended Data Fig. 8. EIF5B depletion reduces cell proliferation without affecting global translation of human lung cancer cells.

a, MTS cell proliferation assay measuring proliferation of H358, H1944, H2030, Calu-6 and H441 cells transfected with control or EIF5B siRNA with n=3 biological replicates. Student’s t-test with Holm-Sidak adjustment was performed per time point to assess statistical significance. ** = p<0.01; *** = p<0.001; **** = p<0.0001. Adjusted p values were as follows for Day 1, 2, 3, 4 and 5 respectively. H358: 0.74, 0.096, 0.064, 0.013 and 0.0001, H1944: 0.495, 0.044, 0.042, 0.0449, 0.018, H2030: 0.801, 0.068, 0.06, 0.039, 0.008, Calu6: 0.57, 0.42, 0.002, 0.016 and 0.008, H441: 0.258, 0.079, 0.01, 0.0002 and 0.012. b, Autoradiography analysis of newly synthesized S-35 labeled proteins in cells from (a). Experiments were performed two independent times for H358 and H1944 cells with similar results. Data from a representative experiment are shown.