Figure 7.

Tyrosine hydroxylase levels are elevated in young DBH-hSNCA Tg mice. TH (green) immunoreactivity is increased in LC neurons (NET; red) at 3 and 14 months of age. A, Representative immunofluorescent images. B, TH IR mean normalized to NET IR mean and expressed as the fold change of Tg from nTg mean ± SEM percentage of IR. C, Western blot analysis of LC neurons from 3-month-old DBH-hSNCA × TH-EGFP mice confirms increased TH abundance. Data graphed as arbitrary densitometric units (AU) were normalized to total protein ± SEM. Representative Western blot images of TH (top) and total protein stain (bottom). Student's t test of nTg and Tg at each age, n = 5–10. Scale bar, 50 µm. *p < 0.05, **p < 0.01.