Figure 8.

Human asyn in LC neurons affects the expression of local inflammatory markers. The expression of astrocytic GFAP (red) is increased in the Tg LC (TH; green) at 24 months of age. A, Representative immunofluorescent images. B, Quantification of the GFAP percentage of IR in the LC as the percentage of ROI graphed as the fold change in Tg IR from nTg mean IR ± SEM. There are fewer Iba1-positive cells (red) in the LC (TH; green) at 14 months. C, Representative immunofluorescent images. D, Microglial count in the LC graphed as the fold change in Tg from nTg Iba1-positive cell count ± SEM. Student's t test of nTg and Tg for each age group. Student's t test of nTg and Tg for each age group. Scale bar, 50 µm. *p < 0.05.