TABLE 1.
Benchmark of the front-end off-target searching (simultaneously searching 10 spacers against the target genome on a platform using i5-3470 at 3.20 GHz)
| Species | Genome size (Mb) | Processing time required for indicated number of mismatchesa |
|||
|---|---|---|---|---|---|
| ≤3 | ≤2 | ≤1 | =0 | ||
| Escherichia coli CDS | 4.66 | 0m1s ± 0.01s | 0m1s ± 0.02s | 0m0s ± 0.01s | 0m0s ± 0.02s |
| Escherichia coli DNA | 4.50 | 0m1s ± 0.01s | 0m0s ± 0.02s | 0m0s ± 0.02s | 0m0s ± 0.01s |
| Saccharomyces cerevisiae CDS | 11.10 | 0m1s ± 0.04s | 0m1s ± 0.03s | 0m1s ± 0.03s | 0m0s ± 0.01s |
| Saccharomyces cerevisiae DNA | 11.79 | 0m1s ± 0.04s | 0m1s ± 0.03s | 0m1s ± 0.03s | 0m0s ± 0.01s |
| Caenorhabditis elegans CDS | 52.00 | 0m6s ± 0.20s | 0m4s ± 0.13s | 0m3s ± 0.19s | 0m2s ± 0.01s |
| Caenorhabditis elegans DNA | 97.24 | 0m12s ± 0.24s | 0m8s ± 0.24s | 0m6s ± 0.26s | 0m4s ± 0.02s |
| Danio rerio CDS | 90.86 | 0m11s ± 0.23s | 0m7s ± 0.04s | 0m5s ± 0.21s | 0m3s ± 0.01s |
| Danio rerio DNA | 1,304.19 | 2m35s ± 3.57s | 1m46s ± 3.12s | 1m13s ± 0.28s | 0m47s ± 0.25s |
| Mus musculus CDS | 98.64 | 0m12s ± 0.41s | 0m8s ± 0.25s | 0m6s ± 0.25s | 0m3s ± 0.01s |
| Mus musculus DNA | 2,642.60 | 5m7s ± 7.03s | 3m33s ± 6.42s | 2m32s ± 6.24s | 1m35s ± 0.05s |
| Homo sapiens CDS | 146.49 | 0m18s ± 0.01s | 0m12s ± 0.30s | 0m8s ± 0.36s | 0m5s ± 0.03s |
| Homo sapiens DNA | 2,994.31 | 5m52s ± 7.89s | 4m2s ± 7.02s | 2m52s ± 7.04s | 1m48s ± 0.20s |
a
m, minutes; s, seconds.