Video 2. FLIP of SEP-Nfasc186 in the distal axon proximal to the axon terminus.

Download video file ^{(3.4MB, mp4)}

SEP-Nfasc186 fluorescence signal is depleted in the axon (arrows) proximal to the ROI (outlined by the box) following FLIP (top) and at the axon terminal itself showing that SEP-Nfasc186 moves retrogradely from and anterogradely to the axon terminus. The lower movie shows no significant bleaching of a control axon during the same acquisition period. Real interframe interval, 2 s. Scale bar, 5 µm.