Fig. 1.

Characterization of anti-CD27 mAb 2B3. a ELISA binding to human CD27. Binding detected with an HRP-labeled goat anti-human IgG (Fc-specific) antibody. b 2B3 blocks CD70 binding to Ramos cells expressing CD27. Ramos cells were incubated with mAbs followed by the addition of CD70-biotin. CD70-biotin was detected with streptavidin-PE. c CD27 agonist activity of 2B3. NFκB reporter 293 cells expressing CD27 were incubated with mAbs. Luciferase production was detected with Luciferase Assay System (Promega) d 2B3 enhances antigen-specific T cell responses to OVA immunization in vivo. huCD27-Tg mice (n = 3 per group) were injected i.p. with 5 mg of OVA together with 50 µg mAbs and spleens were collected 7 days later. ELISPOT assays were performed in triplicate from animals treated with hIgG1, varlilumab (Varli), or 2B3, and cultured in the presence (black bars) or absence (white bars) of SIINFEKL peptide. Shown are the mean number of spot-forming units per spleen (± SD)