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. 2020 May 25;69(10):2125–2137. doi: 10.1007/s00262-020-02610-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — CDX-527 mediated ADCC. Indicated cell lines expressing PD-L1 (a, b) or CD27 (c, d) were mixed with effector reporter cells in the presence of increasing concentrations of CDX-527, 9H9 or 2B3 for 6 h at 37 °C. ADCC activity was measured through NFAT-driven luciferase expression in effector cells. Mean (± SD) relative light units for duplicates in a single experiment are shown. The experiment was repeated with similar results