Figure 5.

Chromosomal periodicities of the growth rate and gene expression. (A) Spectral power of the Fourier-transformed growth rates. The major spectral power at a wavelength of 772 kb is indicated by the broken line in red. (B) Periodograms of the growth rates. The growth rates of the strains lacking either the non-essential genes (knockout strains) or the essential genes (zero growth) are plotted against the BW25113 genome. The mean growth rates of the strains lacking the corresponding genes (including essential and nonessential genes) in every 1-kb sliding window are shown in 100-kb bins by black lines. The periodicities of the highest spectral power (A, red lines) are shown in the curves in red. The upper and bottom panels indicate growth in M63 and LB, respectively. The Fisher’s g test results for the periods in M63 and LB were 0.168 (p = 2e − 182) and 0.146 (p = 1e − 155), respectively. The ori and dif are indicated by broken lines in black. (C) Spectral power of the Fourier-transformed gene expression. The major spectral power at the wavelength of 772 kb is indicated by the broken line in red. (D) Periodograms of gene expression. The expression levels (log₁₀RPKM) are plotted against the BW25113 genome. The mean expression levels of the genes in every 1-kb sliding window are shown as black lines. The periodicities corresponding to the highest spectral power (C, red lines) are shown as the curves in red. The upper and bottom panels indicate the growth in M63 and LB, respectively. The ori and dif are indicated by broken lines in black. The Fisher’s g test results for the periods in M63 and LB were 0.123 (p = 3e − 129) and 0.099 (p = 2e − 102), respectively.