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. 2020 Sep 23;11:4810. doi: 10.1038/s41467-020-18444-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative flow cytometric data illustrating CD107a degranulation by NK-92MI, CD19-4-1BB-CAR, CD19-CD28-CAR, and CD147-CAR after mixing with the medium (control), SK-Hep1, and HepG2. b Quantitative data for the percentage of surface CD107a expression on CD147-CAR-NK-92MI cells upon different stimulations, as indicated. Data represent the mean ± SEM (n = 4). c, d Cytokine TNF-α (c) and IFNγ (d) production by CD147-CAR-NK-92MI, CD19-4-1BB-CAR-NK-92MI, CD19-CD28-CAR-NK-92MI, and wild-type NK-92MI stimulated by different conditions. Data are pooled from four independent experiments. e Representative data showing the percentage of surface CD107a expression on CD147-CAR-NK-92MI cells upon different stimulations, as indicated. Isotype-mouse IgG (IgG) or PBS (vehicle control group) was used, as indicated. f, g Quantitative data for the percentage of surface CD107a staining on CD147-CAR-NK-92MI cells stimulated with CD147⁺ SK-Hep1 and CD147⁺ HepG2 cell lines. Wild-type NK-92MI cells alone and CD147-CAR-NK-92MI cells alone were used as controls, as indicated. Data represent the mean ± SEM of four independent experiments. h Representative data showing the percentage of surface CD107a expression on CD147-CAR-NK-92MI cells stimulated with CD147⁺ wild-type (WT) SK-Hep1 cell line (top panel) and CD147-knockout (CD147^−/−) SK-Hep1 cell line (middle panel). i Quantitative data for the percentage of surface CD107a staining on CD147-CAR-NK-92MI cells stimulated with CD147⁺ SK-Hep1 (WT) and CD147-knockout SK-Hep1 (CD147^−/−) cell lines, respectively. Data represent the mean ± SEM of three independent experiments. j Representative data showing the percentage of surface CD107a expression on CD147-CAR-NK-92MI cells stimulated with a CD147⁺ wild-type (WT) HepG2 cell line (top panel) and a CD147-knockout HepG2 cell line (middle panel). k Quantitative data for the percentage of surface CD107a staining on CD147-CAR-NK-92MI cells stimulated with CD147⁺ HepG2 (WT) and CD147-knockout HepG2 (CD147^−/−) cell lines, respectively. Data represent the mean ± SEM of three separate experiments. Unpaired Student’s t test was employed for all the panels. ***p < 0.001; n.s. not significant.