Fig. 5. Patient-derived Primary CD147-CAR-NK cells specifically kill CD147⁺ tumor cells in vitro.

a, b Representative H&E and IHC staining of liver samples from different stages of one HCC patient. Scale bar, 200 µm. c Diagram of experimental design of HCC sample acquisition from different areas of liver cancer tissues. Briefly, three regions of interest (tumor zone, adjacent zone, and non-tumor zone) were obtained. Primary NK cells were isolated from these zones, indicated by different colors. Scale bar, 2 cm. d Flow cytometry analysis of CD147-CAR⁺ primary NK cells from different zones of liver tissues. e Cytotoxicity of primary CD147-CAR-NK cells was measured by 4-h standard Cr⁵¹ release assays. All results are mean ± SEM. Data are from at least two experiments.