Figure 1.

Experimental assessment of the purity of soluble recombinant human erythrocyte cyt b5. Panel (A) SDS-PAGE electrophoresis of purified cyt b5 on 15% polyacrylamide gels. Samples of purified cyt b5 were loaded into lanes M1, M2, and M3, and low molecular weight markers were loaded into the two lanes labeled as LMW. Panel (B) Absorption spectra of purified cyt b5. The spectrum of oxidized cyt b5 was recorded at 25 °C with 5 μM of purified cyt b5 in (buffer composition: 50 mM phosphate, pH 7). Reduced cyt b5 spectrum was recorded 30 s after the addition of a spatula tip (2–5 mg) of solid sodium dithionite to the cuvette.