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. 2020 Sep 23;6:93. doi: 10.1038/s41420-020-00324-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The intensity of staining for StAR (green signal, a high magnification, c low magnification) and phospho-c-Jun (green signal, b high magnification, d low magnification) are substantially increased and decreased after treatment with hCG and activin-A respectively. In accordance with this the percentage of the cells stained positive for these markers were significantly decreased in the activin-treated cells in comparison to control and hCG-treated ones (e). Nuclei were highlighted in blue with DAPI staining. Oil Red O staining shows neutral lipid droplets in the cells as red dots. Scale bar is 10 μm.