Figure 4: Frequency and specificity of naïve H-2D^bNA_181-190- and H-2D^bNA_181-191-specific CD8+ T cell precursor populations.

Pooled spleen and major peripheral LN samples from naïve B6 mice were stained with H-2D^bNA_181-190 and H-2D^bNA_181-191 tetramers, magnetically enriched for tetramer-binding cells, and then stained with a panel of fluorescently conjugated antibodies for identification of epitope-specific CD8⁺ T cells. (A) Representative dot plot of live, CD8⁺ CD3⁺ CD4⁻ CD11b⁻ CD11c⁻ F4/80⁻ B220⁻ I-Ab⁻ CD44^lo H-2D^bNA_181-190- and H-2D^bNA_181-191-specific CD8⁺ lymphocytes. (B) Number of naïve H-2D^bNA_181-190- and H-2D^bNA_181-191-specific CD8⁺ T cell precursors from the pooled spleen and LNs of individual mice. Data are combined from 3 different experiments (n=4 mice/experiment).