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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Phytother Res. 2020 May 4;34(9):2366–2384. doi: 10.1002/ptr.6687

Figure 1: Nef inhibits the proliferation of cervical cancer cells:

Figure 1:

(A) Chemical structure of the Neferine. (B) Nef inhibits the proliferation of cervical cancer cells (HeLa and SiHa cells) in a dose dependent manner (48 hrs) as determined by MTT cell proliferation assay. (C) 25μM concentration of Nef shows inhibitory concentration (IC50) at 48hrs and the effects of Nef was abrogated by antioxidant (NAC), autophagy inhibitor (3MA) and caspase inhibitor (CI) (Z-VAD-FMK). (D) Cytotoxicity analysis of Nef on normal kidney cells HEK-293. Cells were treated with various concentrations of Nef (0 to 100μM), results indicate no significant cytotoxicity. Data shown as mean ± SD. *p< 0.01 for differences between controls and Nef treatments; #p< 0.01 for differences between Nef 25μM and Nef 25μM plus NAC inhibitor.