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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Phytother Res. 2020 May 4;34(9):2366–2384. doi: 10.1002/ptr.6687

Figure 2: Nef treatment reduces anchorage-independent growth of cervical cancer cells:

Figure 2:

Figure 2:

(A and B) Soft agar assay results showed that Nef significantly inhibit the colony formation ability of cervical cancer cells. Colonies were detected using crystal violet staining. (C) Colonies were counted and represented in a graph showing that the average number of colonies in the control cells versus Nef-treated cells. Arrow indicates stained colonies. *p<0.01 for difference between Nef treatments and controls. Nef treatment induces inhibition of colony formation ability of cervical cancer cells: (D) Clonogenic assay results showed that Nef suppresses the colony forming ability of cervical cancer cells. Colonies were detected using crystal violet staining. (E) Quantified results showing that Nef robustly inhibits the colony forming ability of both HeLa and SiHa cervical cancer cells. #p<0.01 for difference between Nef treatment and control.