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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Phytother Res. 2020 May 4;34(9):2366–2384. doi: 10.1002/ptr.6687

Figure 3: Transwell migration assay:

Figure 3:

Inhibitory effect of Nef on the migration of HeLa and SiHa cells were determined by Transwell migration assay for 48hrs with different concentration of Nef (10, 25 and 50μM). Results showed that Nef significantly inhibit migration of HeLa and SiHa cells (A) Migrated cells to the bottom side of the Trasnwell membrane insert; (C) Migrated cells to the bottom well of the chamber. Figure (B & D) depict the quantified results based on number of migrated cells counted per field. Images were taken by microscope (Olympus-1X73). Scale: 200μm. *p<0.01; **p<0.001 difference between control and treatment.