Fig. 3.

Overexpression of NHE1 does not further enhance neurite outgrowth following RhoA inhibition. a. Representative examples of WT neurons that were either untransfected (WT) or transiently transfected with HA-tagged full-length NHE1 (NHE1) and cultured for 48 h, in the absence or presence of the ROCK inhibitor Y-27632 (25 μM) and/or cariporide (1 μM). NHE1 transfected neurons were identified by anti-HA antibody. b. Neurons treated with Y-27632 exhibited increased neurite outgrowth which was insensitive to cariporide. Quantification of neurite outgrowth in WT and NHE1 transfected neurons, in the absence or presence of Y-27632 and in the absence or presence of cariporide. **P < 0.01, ***P < 0.001