Figure 4.
Hypersensitive response after co-infiltration of Ty-2 gene with tomato yellow leaf curl virus (TYLCV) genes. (A) Pictures of Nicotiana benthamiana leaves co-infiltrated with constructs expressing the Ty-2 or Ty-1 gene of tomato with CP/V1, Rep/C1, or C4 of TYLCV-IL, compared with infiltration of positive control INF1. (B) DAB staining of the same leaves as shown in (A). Leaf 1 shows the co-infiltration with the viral gene CP/V1, leaf 2 with Rep/C1 and leaf 3 with C4. The letters correspond to the following samples: spot A=Ty-2, B= Ty-2+ CP/V1, C= CP/V1, D=Ty-1+ CP/V1, E=INF1, F=Ty-1, G=Ty-2+ Rep/C1, H= Rep/C1, I=Ty-1+ Rep/C1, J=Ty-2+C4, K=C4, L=Ty-1+C4. Note the hypersensitive response (HR) response for spot G=Ty-2+ Rep/C1. (C) Quantification of 3,3’-diaminobenzidine (DAB) staining. Values are means ± SD from three independent experiments.