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. 2020 Sep 10;8:1029. doi: 10.3389/fbioe.2020.01029

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Copyright © 2020 Du, Alifu, Wu, Chen, Wang, Ji, Li, Qian, Xu and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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3PL imaging of ear blood vessels of a mouse treated with PS-PEG@TB NPs at various vertical depths: (A) 25 μm, (B) 75 μm, (C) 100 μm, (D) 125 μm, (E) 150 μm, and (F) 180 μm. (G) A stacked three-photon fluorescence image from a depth of 0 to 180 μm. (H) A 3D reconstructed image showing the distribution of the PS-PEG@TB NPs in the ear blood vessels of the mouse. Scale bar: 100 μm.