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Schematic of the workflow to assess key features of Nif proteins targeted to plant mitochondria. (A) Translational fusions of the MTP to Nif proteins are expressed in N. benthamiana leaf to test preprotein processing and solubility. (B) Scar::Nif protein fusions are expressed in E. coli for function testing by acetylene reduction. MTP, “scar” peptide, Nif not to scale. MTP, mitochondrial targeting peptide; MPP, mitochondrial processing peptidase.
