Figure 3.

Upregulated or downregulated genes and pathways in colorectal tumor-infiltrating CD8⁺ T cells with increasing stages. Genes which their expression levels significantly increase or decrease with increasing stages (from stages I–IV) were identified. Heatmaps representing the Z- score for genes which were upregulated (A) or downregulated (B) with increasing stages. Functional annotations of significantly downregulated and upregulated genes were performed in DAVID web-based tool. Bars show the fold enrichment of functional pathways that were significantly downregulated or upregulated in CD8⁺ TILs with increasing stages (C). Heatmaps showing the Z-score for significantly upregulated or downregulated genes selected based on the functional analysis in panel (C) (D). IFN-γ, interferon-γ; TCR, T cell receptor; TILs, tumor-infiltrating lymphocytes.