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. 2020 Sep 10;11:563735. doi: 10.3389/fpls.2020.563735

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Copyright © 2020 Seifert

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Three variants of type II AG. Structure (A) was determined from the synthetic AGP (Ala-Hyp)51-EGFP expressed in and secreted from Nicotiana tabacum Bright Yellow 2 suspension cells (Tan et al., 2004). Among other techniques, the Hyp-glycan was analyzed using nuclear magnetic resonance spectrometry. Structure (B) is one of many possible models derived from enzymatic fragmentation and electrophoretic as well as mass spectrometric analysis of AGP bulk-purified from A. thaliana leaves (Tryfona et al., 2012). Stucture (C) shows a type II AG sub-structure linked to APAP1/AGP57C and to arabinoxylan and pectic rhamnogalacturonan I (Tan et al., 2013).