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. 2020 Sep 10;8:569406. doi: 10.3389/fbioe.2020.569406

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Copyright © 2020 Sato, Kishida, Nakano, Hirata and Tanaka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Metabolic engineering of Corynebacterium glutamicum for shikimic acid production. The blue X indicates deletion of the nagD, qsuD, qsuB, qsuD, aroK, and pta genes. Genes involving shikimic acid synthesis pathways are indicated in red. G6P, glucose-6-phosphate; GAP, glyceraldehyde-3-phosphate; DHAP, 1,3-dihydroxyacetone phosphate; DHA, 1,3-dihydroxyacetone; PEP, phosphoenolpyruvate; PYR, pyruvate; OAA, oxaloacetate; Ru5P, ribulose-5-phosphate; E4P, erythrose 4-phosphate; DAHP, 3-deoxy-D-arabinoheptulosonate-7-phosphate; DHQ, 3-de-hydroquinate; DHS, 3-dehydroshikimate; PCA, protocatechuate; aroG, DAHP synthase; aroB, DHQ synthase; aroD, DHQ dehydratase; aroE1, aroE3, shikimate dehydrogenase; aroK, shikimate kinase; qsuB, DHS dehydratase; qsuD, QA/shikimate dehydrogenase; gnd, 6-phosphogluconate dehydrogenase; BGL, beta-glucosidase from Thermobifida fusca YX.