TABLE 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Genotype | Source or reference |
| Escherichia coli | ||
| Nova Blue | endA1 hsdR17(rK12-mK12+) supE44 thi-I gyrA96 relA1 lac recA1/F’ [proAB+ lacIq ZΔM15:Tn10(Tet r)]; used for gene cloning. | Novagen |
| SCS110 | rpsL (Str1) thr leu endA thi-l lacY galK galT ara tonA tsx dam dcm supE44Δ(lac-proAB) [F’ traD36 proAB laclqZΔM15] | STRATAGENE |
| Corynebacterium glutamicum | ||
| ATCC13032 | Wild-type strain | ATCC |
| SA-1 | ATCC13032 + ΔcglMRR (cg1996-cg1998), ΔqsuD,ΔqsuB,ΔnagD,ΔaroK | This study |
| SA-2 | SA-1 + gnd S361F | This study |
| SA-3 | SA-2 + aroK:PH36-aroG | This study |
| SA-4 | SA-2 + aroK:PH36-aroB | This study |
| SA-5 | SA-2 + aroGgtg→atg,Δcg2392 | This study |
| SA-6 | SA-5 + aroK:PH36-aroB | This study |
| SA-7 | SA-5 + aroK:PH36-aroB, pta:PH36-aroG | This study |
| SA-8 | SA-7 + Δldh | This study |
| SA-9 | SA-7 + ldh:PH36-aroE1 | This study |
| SA-10 | SA-7 + ldh:PH36-aroE3 | This study |
| Plasmids | ||
| pCCS | E. coli–C. glutamicum shuttle vector for control, KmR | Matsuura et al., 2019 |
| pCC-H36-cgR0949-Tfu0937 | pCCS derivative carrying sequences encoding the CgR0949 secretion signal fused to T. fusca Tfu0937 under the control of the H36 promoter | Matsuura et al., 2019 |
| pCC-PH36-aroG | pCCS containing aroG under the control of the H36 promoter | This study |
| pCC-PH36-aroB | pCCS containing aroB under the control of the H36 promoter | This study |
| pCC-PH36-qsuC | pCCS containing qsuC under the control of the H36 promoter | This study |
| pCC-PH36-aroE1 | pCCS containing aroE1 under the control of the H36 promoter | This study |
| pCC-PH36-aroE3 | pCCS containing aroE3 under the control of the H36 promoter | This study |
| pCC-PH36-tal-tkt | pCCS containing tal and tkt (cg1774-cg1776, NCgl1512-NCgl1513) under the control of the H36 promoter | This study |
| pCC-PH36-pck | pCCS containing pck under the control of the H36 promoter | This study |
| pK18mobsacB | sacB, lacZ, KmR, MCS, mobilizable vector, enables selection/counter-selection for integration/excision in C. glutamicum | ATCC |
| pK18-gndS361F | pK18mobsacB derivative for substitution of gndS361F | This study |
| pK18-ΔqsuD | pK18mobsacB derivative for qsuD deletion | This study |
| pK18-ΔqsuB | pK18mobsacB derivative for qsuB deletion | This study |
| pK18-ΔnagD | pK18mobsacB derivative for nagD deletion | This study |
| pK18-ΔaroK | pK18mobsacB derivative for aroK deletion | This study |
| pK18-Δldh | pK18mobsacB derivative for ldh deletion | This study |
| pK18-ΔaroK-PH36aroB | pK18mobsacB derivative for insertion of aroB expression cassette under the control of H36 promoter into aroK locus | This study |
| pK18-Δpta-PH36aroG | pK18mobsacB derivative for insertion of aroG expression cassette under the control of H36 promoter into pta locus | This study |
| pK18-Δldh-PH36aroE1 | pK18mobsacB derivative for insertion of aroE1 expression cassette under the control of H36 promoter into ldh locus | This study |
| pK18-Δldh-PH36aroE3 | pK18mobsacB derivative for insertion of aroE3 expression cassette under the control of H36 promoter into ldh locus | This study |