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. 2020 Sep 10;14:570917. doi: 10.3389/fncel.2020.570917

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Copyright © 2020 Li, Wing, Wang, Luu, Bender, Chen, Wang, Lu, Nguyen Tran, Young, Wong, Pébay, Cook, Hung, Liu and Hewitt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AAV7m8 mediated delivery of CRISPR/Cas to the mouse retina in vivo. (A) Schematic diagram of in vivo experiment. Mice were sacrificed 5 months after intravitreal injection. (B) Representative cross section image from retina co-transduced with AAV7m8-CRISPR/Cas and its selected YFP sgRNA. Mouse ID 29, right eye Robust AAV7m8 transductions in the retina were found. Scale bar: 200 μm. Images were taken by a Zeiss spinning disk confocal microscope. (C) Representative retinal whole-mount images from a mouse eye receiving AAV7m8-CRISPR/Cas and its selected YFP sgRNA. Mouse ID 76, right eye. Scale bar: 500 μm. Images were taken using an Olympus Slide Scanner. Please see Supplementary Figure S3 for representative cross-sectional images with higher magnification.