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. 2020 Sep 10;13:161. doi: 10.3389/fnmol.2020.00161

Figure 3.

Figure 3

Expression of presynaptic (synapsin I; green) and postsynaptic (PSD95; red) markers at day in vitro (DIV) 14 in Fmr1 knockout (KO) MSNs transfected with either green fluorescent protein (GFP) alone, or various forms of EGFP-tagged FMRP (A; scale bars are 10 μm). Cells transfected with ΔRGG-FMRP have a higher density of synapsin than those transfected with wt- or I304N-FMRP (B; one-way ANOVA). Cells transfected with wt-FMRP had significantly decreased density of PSD95 puncta, while those transfected with I304N- or ΔRGG-FMRP did not differ from GFP (KO) controls (C; one-way ANOVA). Cells transfected with ΔRGG-FMRP had a greater density of colocalized synapsin and PSD95 puncta compared to those transfected with I304N-FMRP and GFP (KO) controls (D; one-way ANOVA). Significant Bonferroni comparisons are indicated (*p < 0.05, **p < 0.01, ****p < 0.0001); data shown are mean ± SEM.