Figure 1.
Characterization of CFSs in HCT116 and RPE1 Epithelial Cells
(A) Representative metaphase spreads (reverse DAPI banding) from RPE1 (left) and HCT116 (right) cell lines, showing CFS fragility (red arrows) after aphidicolin (APH) treatment (top); bottom, extreme chromosomal defects in HCT116 cells; Scale bar, 5 μm.
(B) Ideograms showing most frequent APH-dependent common fragile site locations in RPE1 and HCT116 epithelial cells, cytogenetically scored by DAPI banding. CFSs specific to HCT116 cells (blue), RPE1 (green), and both (mauve) are indicated.
(C) Length of largest transcript (top) and GC content (bottom) at sites fragile in HCT116 (blue), RPE1 (green), or both cell lines (mauve).
(D) Left, genome-wide GC (percentage in 0.5-Mb windows) density plot with GC density at CFSs in HCT116 (blue), RPE1 (green) or both cell lines (mauve). Right, genome-wide gene-length (NCBI genes) density plot with gene length of genes encompassed within CFSs in HCT116 (blue), RPE1 (green), or both cell lines (mauve).