REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CAP-H	Bethyl	Cat# A300-603A; RRID:AB_2150006
CAP-D3	Bethyl	Cat# A300-604A; RRID:AB_2298269
GAPDH	Cell Signaling	Cat# 2118; RRID:AB_561053
H3S10pclone CMA313	Gift from Hiroshi Kimura	N/A
Rabbit anti-SMC2	Kumiko Samejima	N/A
Texas Red anti-mouse	Jackson Immuno Research	Cat# 715-585-150; RRID:AB_2340854
FITC anti-rabbit	Jackson Immuno Research	Cat# 711-095-152; RRID:AB_2315776
FITC avidin	Vector Labs	Cat# A-2011; RRID:AB_2336456
Biotin anti-avidin	Vector Labs	Cat# BA-0300; RRID:AB_2336108
Texas Red anti-sheep	Vector Labs	Cat# TI-6000; RRID:AB_2336219
Anti-dig rhodamine	Roche	Cat# 11207750910; RRID:AB_514501
Chemicals, Peptides, and Recombinant Proteins
Aphidicolin	Calbiochem	38966-21-1
Colcemid	Life Technologies	15210-040
Calyculin	Simga	C5552-10UG
biotin-16-dUTP	Roche	11093070910
digoxigenin-11-dUTP	Roche	11093088910
Alexa Fluor 488 Azide	Thermo Fisher	A10266
DNA Polymerase I	Invitrogen	18010025
RNase A	Invitrogen	12091039
DNaseI	Roche	4716728001
Critical Commercial Assays
Click-it Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit	Invitrogen	C10634
Experimental Models: Cell Lines
hTERT RPE1	ATCC	CRL-4000
HCT 116	ATCC	CCL-247
SMC2-AID-mClover HCT 116	This study	N/A
CAPH-AID-mCherry HCT 116	Takagi et al.	N/A
CAPH2-AID-mCherry HCT 116	Takagi et al.	N/A
Recombinant DNA
BAC: RP11-357C16	BACPAC Resources	N/A
BAC: RP11-452B11	BACPAC Resources	N/A
BAC: RP11-482A14	BACPAC Resources	N/A
BAC: RP11-44E15	BACPAC Resources	N/A
BAC: RP11-624N7	BACPAC Resources	N/A
Fosmid: G248P86197B3	BACPAC Resources	N/A
Fosmid: G248P83504C1	BACPAC Resources	N/A
BAC: RP11-436I1	BACPAC Resources	N/A
BAC: RP11-236P10	BACPAC Resources	N/A
BAC: RP11-56K5	BACPAC Resources	N/A
Fosmid: G248P8183F5	BACPAC Resources	N/A
Fosmid: G248P89337E4	BACPAC Resources	N/A
BAC: RP11-27C2	BACPAC Resources	N/A
BAC: RP11-1053C2	BACPAC Resources	N/A
BAC: RP11-44A17	BACPAC Resources	N/A
BAC: RP11-351L22	BACPAC Resources	N/A
BAC: RP11-479E18	BACPAC Resources	N/A
BAC: RP11-915N9	BACPAC Resources	N/A
BAC: RP11-6L24	BACPAC Resources	N/A
BAC: RP11-946L12	BACPAC Resources	N/A
BAC: RP11-153D1	BACPAC Resources	N/A
BAC: RP11-126P21	BACPAC Resources	N/A
BAC: RP11-795A13	BACPAC Resources	N/A
BAC: RP11-412C14	BACPAC Resources	N/A
Software and Algorithms
ImageJ	Open Source	N/A