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. 2020 Sep 10;10:1756. doi: 10.3389/fonc.2020.01756

FIGURE 4.

FIGURE 4

Effect of Tan IIA and DDP alone and in combination on cell cycle arrest. The percentage of cells in G1, S or G2/M phase in HK (A) or K180 cells (C) treated with 6 μM Tan IIA, 3 μM DDP alone and 1 μM DDP and 2 μM Tan IIA in combination for 24 h. Data represent the cell population of cell cycle arrest of HK (B) and K180 (D) cells. The 3D crystal structure of human P21 with an endogenous ligand (PDB-ID: 2zvw) (E). Ten random poses of Tan IIA docked into the active site of 2zvw (F). The binding modes of Tan IIA in P21: at least five residue involved in the interactions in ten random poses, ASP 156, ALA 208, ARG 210 (H-bonds) and ALA 208, ARG 210 (π-π interaction) (G). Ten random poses of DDP docked into the same active site of 2zvw (H). The binding modes of DDP in P21: at least two residue involved in the interactions in ten random poses, ASP 156, ARG 210 (H-bonds) (I). Protein expression levels of Cyclin D1, p21, p27, and GAPDH of HK (J) and K180 (K) cells following 24 h treatment with 6 μM Tan IIA, 3 μM DDP alone and 1 μM DDP and 2 μM Tan IIA in combination. Histograms represent the relative gray value of the related proteins measured by Image J (L,M). All data are depicted as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, or ****P < 0.0001 versus the control group.