FIGURE 2.

Accumulation of mtDNA mutations in PolgA mutator mice affects fertility in a sex‐ and age‐dependent manner. (a, b) The mean litter sizes of the first, second, and third litters per breeding pair at 7–8 weeks of age as indicated in the legends. The breeding pairs were generated as shown in a. The litter sizes were shown in b (n = 6 pair in Mut/Mut***×Mut/Mut*** breeding group, and n = 11 pair in the other breeding pairs). The values of p1, p2, and p3 were calculated using litters 1, 2, and 3 from WT/WT×WT/WT breeding pair as control. (c) The mean number of pups in the first litters per breeding pair at 3 months of age as indicated in the legends (n = 6 pair in Mut/Mut***×Mut/Mut*** breeding group, and n = 8 pair in the other breeding groups). (d) The mean number of pups in the first litters per WT/Mut**×WT/Mut** breeding pair at generations F1 to F3 as indicated in the legends (n = 10 pair in F1, n = 8 pair in F2 and n = 6 pair in F3). Error bars are SD, and p‐values were calculated using one‐way ANOVA test