Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 23;10:15519. doi: 10.1038/s41598-020-72452-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(A) Uptake of different radiolabelled, fluorescent conjugates normalized to uptake in iron-depleted media of the respective compound. Grey bars represent control conditions with TAFC. Blocking with [Fe]TAFC reduces cellular uptake due to competition with the natural substrate of the MirB transporter. Fe (+) represents iron-replete conditions, which decreases biosynthesis of the MirB transporter. (B) Competition assay of [⁶⁸Ga]Ga‐TAFC blocked with iron-containing fluorophore compounds in iron-depleted minimal medium. Values that are lower compared to the [⁶⁸Ga]Ga‐TAFC uptake of control (grey bars), indicate a specific interaction of the fluorophore conjugate with the MirB transporter. Data of [Fe]DAFC-Cy5 are adapted from reference¹⁸.