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. 2020 Sep 23;10:15519. doi: 10.1038/s41598-020-72452-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Internalisation and subcellular localisation pattern of fluorescent siderophore conjugates in A. fumigatus and A. terreus. [Fe]DAFC-FITC, -Ocean Blue, -NBD and –BODIPY are specifically internalised by A. fumigatus—but not by A. terreus—and accumulate in the cytoplasm, vacuoles and internal membranes, respectively. [Fe]DAFC-SiR and –Cy5 show pronounced internalisation into A. fumigatus, however, do leak into A. terreus by a MirB-independent mechanism. Interestingly, [Fe]DAFC-TAMRA is not internalised to detectable levels by either species. “Dye alone” controls—using identical molarities, incubation and imaging conditions—showed that only BODIPY, SiR and Cy5 are able to rapidly cross the cell wall matrix and plasma membrane barrier. Scale bars, 10 μm.