Expression patterns of HLA-I and the NKp30/VAV2/MAPK3/IL-12 pathway in GC cell lines. (a) qPCR detected relative CNV of HLA-A, B and C in GC cell lines. The value of HLA-I is the sum value of HLA-A, -B and -C. Owing to the copy number of HLA-A, -B was not changed in AGS cells, the relative copy number of HLA-A, -B was compared with AGS cells; for HLA-C, as it was lost in AGS cells, the relative copy number of HLA-C was compared with BGC823 cells. (b) Western blot analysis of NK cell cytotoxicity-related proteins in GC cell lines with high and low tumorigenic capacities.