Figure 9.

ROS content measurement in progesterone-treated RINm5F cells. Cells were preincubated with 40 μM vitamin E or 50 μM vitamin C for 2 h, and then with 0.1, 1.0, or 100 μM progesterone for 3 (a), 6 (b), or 24 h (c). ROS content was measured by DCFDA probe (10 μM) during 60 min. Data are expressed as mean ± SEM of DCF formation (AUF/min) of three experiments in quadruplicates, after normalization to the number of viable cells in each sample. ^∗ indicates difference in comparison to control without progesterone. $ and $$ indicate difference in comparison to the treatment without antioxidants (ρ < 0.05 and ρ < 0.001, respectively). PG: progesterone; Vit E: vitamin E; Vit C: vitamin C.