Figure 4.

Bml 284 reversed chrysophanol attenuated NF-κB and β-catenin nuclear translocation. (a) FaDu cells cultured and treated as above were collected, and cytosolic and nuclear fractions were isolated as described in Materials and Methods. Western blot analysis was performed to detect the subcellular localization of p65 and β-catenin using an antibody against the NF-κB subunit p65. β-actin was used as a cytosolic marker, and fibrillarin served as a nuclear marker. (b) Densitometric analysis of all samples normalized against the level of total protein. The images are representative of the results of three independent experiments. All data are presented as the mean ± SD. ^∗P < 0.05. Cho indicates chrysophanol.